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The Fire Fighter Cancer Cohort Study: Protocol for a Longitudinal Occupational Cohort Study
JMIR Res Protoc 2025;14:e70522
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Before the main analyses, independent samples t tests (2-tailed, paired) will be conducted on the baseline demographic variables to identify any significant differences between the 2 groups. Any variables that show statistically significant differences will be included as covariates in the subsequent main analyses. It is expected that the treatment group will demonstrate improved emotion regulation and reduced mood symptoms and loneliness following the music intervention, compared with the control group.
JMIR Res Protoc 2025;14:e67764
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Cells in the 5-m L culture tubes will be stimulated with phorbol myristate acetate (Sigma Aldrich) and ionomycin (Sigma Aldrich) in the presence of Golgi Stop (Becton Dickinson) for 4 hours in 37 °C under a 5% CO2 environment to stimulate T-effector cells. Following the incubation periods, the supernatant from cortisol-stimulated cells will be collected and stored at –80 °C until assayed for interleukin (IL)-6, IL-10, IL-8, and IL-4 by using a commercial enzyme-linked immunosorbent assay.
JMIR Res Protoc 2025;14:e66439
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